Coding

Part:BBa_K2807001:Design

Designed by: Ravichandran Divyapoorani   Group: iGEM18_NUS_Singapore-Sci   (2018-09-30)


dPspCas13b


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2884
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1787
    Illegal BamHI site found at 839
    Illegal BamHI site found at 3271
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1520
    Illegal AgeI site found at 2396
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Original sequence had four Biobrick incompatible PstI sites, which were mutated from CTGCAG to CTTCAG at nucleotides 237, 633, 1023, and 2445 via sequential Q5 site-directed mutagenesis.

Source

Addgene Plasmid 103865, Feng Zhang's Lab

References